Bioinformatics Tutorial

《生物信息学实践教程》

Teaching Philosophy

🎦 Study and Practice | 格物致知知行合一

"Tell me and I forget. Teach me and I remember. Involve me and I learn." - Benjamin Franklin

We teach professional skills in bioinformatics. These skills are not just running software. They will give you the freedom of exploring various real data.

Courses

本书主要用于清华大学本科生课《生物信息学》和博士生课《生物信息学实践》

Aim

写在前面的话

相对于过去，突然地，我们发现数据不是太少而是太多，信息不是匮乏而是繁杂，新一代人的重要能力是“鉴别”和“挖掘”。

对生物信息学的工作而言，最重要的、最有用的基本工具和技能过去一直是，我相信很长一段时间也会始终是：

google
wikipedia
知乎

We aim to teach basic data skills that give you freedom.

Running bioinformatics software isn’t all that difficult, doesn’t take much skill, and it doesn’t embody any of the significant challenges of bioinformatics.…These data skills give you freedom…
I believe these two qualities — reproducibility and robustness.
So what is a reproducible bioinformatics project? At the very least, it’s sharing your project’s code and data.
In wet lab biology, when experiments fail, it can be very apparent, but this is not always true in computing. Electrophoresis gels that look like Rorschach blots rather than tidy bands clearly indicate something went wrong. Unfortunately, without prior expectations, it can be quite difficult to distinguish good results from bad results.
The easy way to ensure everything is working properly is to adopt a cautious attitude , and check everything between computational steps.
You will almost certainly have to rerun an analysis more than once.
Write Code for Humans, Write Data for Computers
Use Existing Libraries Whenever Possible
Treat Data as Read-Only
Document Everything (-- Too geeky?) Just as a well-organized laboratory makes a scientist’s life easier, a well-organized and well-documented project makes a bioinformatician’s life easier.
-- <<Bioinformatics Data Skills>>

Courses required before this tutorial

基本生物课程：如《遗传学》和/或《分子生物学》
基本统计课程：如《概率论》和/或《生物统计》
基本数学课程：如《微积分》和《线性代数》
基本计算机课程：如《Linux》和《C或Python语言》

Major Authors

Yumin Zhu, Gang Xu, Zhuoer Dong, Yinghui Chen, Meifeng Zhou, Xupeng Chen, Xiaocheng Xi, Xi Hu, Jingyi Cao, Xiaofan Liu, Weihao Zhao, Siqi Wang and Zhi J. Lu

Section	Major Authors
Part I. Basic Skills
1.Setup	Zhi John Lu
1.1 Docker	Gang Xu/Yunfan Jin
1.2 Cluster	Gang Xu/Xiaofan Liu/Yunfan Jin
2.Linux	Zhi John Lu
2.1 Basic Command	Xi Hu
2.2 Practice Guide	Xi Hu/Zhuoer Dong
2.3 Linux Bash	Gang Xu
3.R
3.1 R Basics	Zhuoer Dong
3.2 Plot with R	Xiaochen Xi/Zhuoer Dong
4.Python	Yuhuan Tao

PART II. BASIC ANALYSES
1.Blast	Gang Xu
2.Conservation Analysis	Xi Hu
3.Function Analysis
3.1 GO	Gang Xu
3.2 KEGG	Gang Xu
3.3 GSEA	Zhuoer Dong
4.Clinical Analysis
4.1 Survival Analysis	Xiaochen Xi/Yumin Zhu

Part III. NGS DATA ANALYSES
1.Mapping	Meifeng Zhou/Yumin Zhu
1.1 Genome Browser	Xiaofan Liu/Shang Zhang
1.2 bedtools and samtools	Xiaofan Liu/Yunfan Jin
2.RNA-seq
2.1 Expression Matrix	Xiaofan Liu
2.2 Differential Expression with Cufflinks	Meifeng Zhou/Shang Zhang
2.3 Differential Expression with DEseq2 and edgeR	Xinzhe Ni/Shang Zhang
2.4 Alternative Splicing	Zhuoer Dong/Shang Zhang
3.ChIP-seq	Jingyi Cao/Xiaofan Liu
4.Motif
4.1 Sequence Motif	Yumin Zhu
4.2 Structure Motif	Yumin Zhu
5.Network
5.1 Co-expression Network	Xiaochen Xi
5.2 miRNA Targets	Yumin Zhu
5.3 CLIP-seq(RNA-Protein Interactions)	Yumin Zhu/Xiaofan Liu
6.RNA Regulation Analyses
6.1 Alternative Splicing	Zhuoer Dong/Shang Zhang
6.2 APA (Alternative Polyadenylation)	Yumin Zhu
6.3 Chimeric RNA	Yinghui Chen
6.4 RNA Editing	Yumin Zhu
6.5 SNV/INDEL	Yinghui Chen
6.6 RNA Modification
6.7 RNA Degradation
6.8 Translation:Ribo-seq	Yumin Zhu/Weihao Zhao/Xiaofan Liu
6.9 RNA Structure	Yumin Zhu/Xiaofan Liu

Part IV. MACHINE LEARNING
1.Machine Learning Basics	Xiaofan Liu/Xupeng Chen/Zhi John Lu
1.1 Data Pre-processing	Xinzhe Ni/Xiaofan Liu
1.2 Data Visualization & Dimension Reduction	Xinzhe Ni/Xiaofan Liu
1.3 Feature Extraction and Selection	Xinzhe Ni/Xiaofan Liu
1.4 Machine Learning Classifiers/Models	Xinzhe Ni/Xiaofan Liu
1.5 Performance Evaluation	Xiaofan Liu
2.Machine Learning with R	Xupeng Chen/Xiaofan Liu
3.Machine Learning with Python	Xupeng Chen/Xiaofan Liu

Part V. QUIZ
1.Precision Medicine - exSEEK	Xiaofan Liu/Xupeng Chen
2.RNA Regulation - RiboShape	Xiaofan Liu/Yizi Zhao
3.Single Cell Data Analysis	Yu Li

Appendix
Appendix I. Keep Learning	Zhi John Lu
Appendix II. Databases & Servers	Yumin Zhu
Appendix III. How to Backup	Gang Xu/Zhi John Lu
Appendix IV. Teaching Materials	Gang Xu/Xiaofan Liu/Zhi John Lu
Appendix V. Software and Tools	Yumin Zhu

Section

Major Authors

Part I. Basic Skills

1.Setup

Zhi John Lu

1.1 Docker

Gang Xu/Yunfan Jin

1.2 Cluster

Gang Xu/Xiaofan Liu/Yunfan Jin

2.Linux

Zhi John Lu

2.1 Basic Command

Xi Hu

2.2 Practice Guide

Xi Hu/Zhuoer Dong

2.3 Linux Bash

Gang Xu

3.R

3.1 R Basics

Zhuoer Dong

3.2 Plot with R

Xiaochen Xi/Zhuoer Dong

4.Python

Yuhuan Tao

PART II. BASIC ANALYSES

1.Blast

Gang Xu

2.Conservation Analysis

Xi Hu

3.Function Analysis

3.1 GO

Gang Xu

3.2 KEGG

Gang Xu

3.3 GSEA

Zhuoer Dong

4.Clinical Analysis

4.1 Survival Analysis

Xiaochen Xi/Yumin Zhu

Part III. NGS DATA ANALYSES

1.Mapping

Meifeng Zhou/Yumin Zhu

1.1 Genome Browser

Xiaofan Liu/Shang Zhang

1.2 bedtools and samtools

Xiaofan Liu/Yunfan Jin

2.RNA-seq

2.1 Expression Matrix

Xiaofan Liu

2.2 Differential Expression with Cufflinks

Meifeng Zhou/Shang Zhang

2.3 Differential Expression with DEseq2 and edgeR

Xinzhe Ni/Shang Zhang

2.4 Alternative Splicing

Zhuoer Dong/Shang Zhang

3.ChIP-seq

Jingyi Cao/Xiaofan Liu

4.Motif

4.1 Sequence Motif

Yumin Zhu

4.2 Structure Motif

Yumin Zhu

5.Network

5.1 Co-expression Network

Xiaochen Xi

5.2 miRNA Targets

Yumin Zhu

5.3 CLIP-seq(RNA-Protein Interactions)

Yumin Zhu/Xiaofan Liu

6.RNA Regulation Analyses

6.1 Alternative Splicing

Zhuoer Dong/Shang Zhang

6.2 APA (Alternative Polyadenylation)

Yumin Zhu

6.3 Chimeric RNA

Yinghui Chen

6.4 RNA Editing

Yumin Zhu

6.5 SNV/INDEL

Yinghui Chen

6.6 RNA Modification

6.7 RNA Degradation

6.8 Translation:Ribo-seq

Yumin Zhu/Weihao Zhao/Xiaofan Liu

6.9 RNA Structure

Yumin Zhu/Xiaofan Liu

Part IV. MACHINE LEARNING

1.Machine Learning Basics

Xiaofan Liu/Xupeng Chen/Zhi John Lu

1.1 Data Pre-processing

Xinzhe Ni/Xiaofan Liu

1.2 Data Visualization & Dimension Reduction

Xinzhe Ni/Xiaofan Liu

1.3 Feature Extraction and Selection

Xinzhe Ni/Xiaofan Liu

1.4 Machine Learning Classifiers/Models

Xinzhe Ni/Xiaofan Liu

1.5 Performance Evaluation

Xiaofan Liu

2.Machine Learning with R

Xupeng Chen/Xiaofan Liu

3.Machine Learning with Python

Xupeng Chen/Xiaofan Liu

Part V. QUIZ

1.Precision Medicine - exSEEK

Xiaofan Liu/Xupeng Chen

2.RNA Regulation - RiboShape

Xiaofan Liu/Yizi Zhao

3.Single Cell Data Analysis

Yu Li

Appendix

Appendix I. Keep Learning

Zhi John Lu

Appendix II. Databases & Servers

Yumin Zhu

Appendix III. How to Backup

Gang Xu/Zhi John Lu

Appendix IV. Teaching Materials

Gang Xu/Xiaofan Liu/Zhi John Lu

Appendix V. Software and Tools

Yumin Zhu

Contact Us

Lu Lab 鲁志实验室
School of Life Sciences, Tsinghua University, Beijing, China
e-mail: lulab1 AT tsinghua.edu.cn
Homepage: www.ncRNAlab.org ( lulab.life.tsinghua.edu.cn )
Software: software.ncRNAlab.org
Courses: courses.ncRNAlab.org
Books: book.ncRNAlab.org ( bioinfo.gitbook.io )
Docs: docs.ncRNAlab.org ( lulab.gitbook.io | lulab.github.io )

Copyright

https://www.apache.org/licenses/LICENSE-2.0

2016-2024年于清华园

Last updated 3 months ago

Aim

写在前面的话

相对于过去，突然地，我们发现数据不是太少而是太多，信息不是匮乏而是繁杂，新一代人的重要能力是“鉴别”和“挖掘”。

对生物信息学的工作而言，最重要的、最有用的基本工具和技能过去一直是，我相信很长一段时间也会始终是：

google
wikipedia
知乎

We aim to teach basic data skills that give you freedom.

Running bioinformatics software isn’t all that difficult, doesn’t take much skill, and it doesn’t embody any of the significant challenges of bioinformatics.…These data skills give you freedom…
I believe these two qualities — reproducibility and robustness.
So what is a reproducible bioinformatics project? At the very least, it’s sharing your project’s code and data.
In wet lab biology, when experiments fail, it can be very apparent, but this is not always true in computing. Electrophoresis gels that look like Rorschach blots rather than tidy bands clearly indicate something went wrong. Unfortunately, without prior expectations, it can be quite difficult to distinguish good results from bad results.
The easy way to ensure everything is working properly is to adopt a cautious attitude , and check everything between computational steps.
You will almost certainly have to rerun an analysis more than once.
Write Code for Humans, Write Data for Computers
Use Existing Libraries Whenever Possible
Treat Data as Read-Only
Document Everything (-- Too geeky?) Just as a well-organized laboratory makes a scientist’s life easier, a well-organized and well-documented project makes a bioinformatician’s life easier.

-- <<Bioinformatics Data Skills>>

Major Authors

Yumin Zhu, Gang Xu, Zhuoer Dong, Yinghui Chen, Meifeng Zhou, Xupeng Chen, Xiaocheng Xi, Xi Hu, Jingyi Cao, Xiaofan Liu, Weihao Zhao, Siqi Wang and Zhi J. Lu

Section	Major Authors
Part I. Basic Skills
1.Setup	Zhi John Lu
1.1 Docker	Gang Xu/Yunfan Jin
1.2 Cluster	Gang Xu/Xiaofan Liu/Yunfan Jin
2.Linux	Zhi John Lu
2.1 Basic Command	Xi Hu
2.2 Practice Guide	Xi Hu/Zhuoer Dong
2.3 Linux Bash	Gang Xu
3.R
3.1 R Basics	Zhuoer Dong
3.2 Plot with R	Xiaochen Xi/Zhuoer Dong
4.Python	Yuhuan Tao

PART II. BASIC ANALYSES
1.Blast	Gang Xu
2.Conservation Analysis	Xi Hu
3.Function Analysis
3.1 GO	Gang Xu
3.2 KEGG	Gang Xu
3.3 GSEA	Zhuoer Dong
4.Clinical Analysis
4.1 Survival Analysis	Xiaochen Xi/Yumin Zhu

Part III. NGS DATA ANALYSES
1.Mapping	Meifeng Zhou/Yumin Zhu
1.1 Genome Browser	Xiaofan Liu/Shang Zhang
1.2 bedtools and samtools	Xiaofan Liu/Yunfan Jin
2.RNA-seq
2.1 Expression Matrix	Xiaofan Liu
2.2 Differential Expression with Cufflinks	Meifeng Zhou/Shang Zhang
2.3 Differential Expression with DEseq2 and edgeR	Xinzhe Ni/Shang Zhang
2.4 Alternative Splicing	Zhuoer Dong/Shang Zhang
3.ChIP-seq	Jingyi Cao/Xiaofan Liu
4.Motif
4.1 Sequence Motif	Yumin Zhu
4.2 Structure Motif	Yumin Zhu
5.Network
5.1 Co-expression Network	Xiaochen Xi
5.2 miRNA Targets	Yumin Zhu
5.3 CLIP-seq(RNA-Protein Interactions)	Yumin Zhu/Xiaofan Liu
6.RNA Regulation Analyses
6.1 Alternative Splicing	Zhuoer Dong/Shang Zhang
6.2 APA (Alternative Polyadenylation)	Yumin Zhu
6.3 Chimeric RNA	Yinghui Chen
6.4 RNA Editing	Yumin Zhu
6.5 SNV/INDEL	Yinghui Chen
6.6 RNA Modification
6.7 RNA Degradation
6.8 Translation:Ribo-seq	Yumin Zhu/Weihao Zhao/Xiaofan Liu
6.9 RNA Structure	Yumin Zhu/Xiaofan Liu

Part IV. MACHINE LEARNING
1.Machine Learning Basics	Xiaofan Liu/Xupeng Chen/Zhi John Lu
1.1 Data Pre-processing	Xinzhe Ni/Xiaofan Liu
1.2 Data Visualization & Dimension Reduction	Xinzhe Ni/Xiaofan Liu
1.3 Feature Extraction and Selection	Xinzhe Ni/Xiaofan Liu
1.4 Machine Learning Classifiers/Models	Xinzhe Ni/Xiaofan Liu
1.5 Performance Evaluation	Xiaofan Liu
2.Machine Learning with R	Xupeng Chen/Xiaofan Liu
3.Machine Learning with Python	Xupeng Chen/Xiaofan Liu

Part V. QUIZ
1.Precision Medicine - exSEEK	Xiaofan Liu/Xupeng Chen
2.RNA Regulation - RiboShape	Xiaofan Liu/Yizi Zhao
3.Single Cell Data Analysis	Yu Li

Appendix
Appendix I. Keep Learning	Zhi John Lu
Appendix II. Databases & Servers	Yumin Zhu
Appendix III. How to Backup	Gang Xu/Zhi John Lu
Appendix IV. Teaching Materials	Gang Xu/Xiaofan Liu/Zhi John Lu
Appendix V. Software and Tools	Yumin Zhu

Section

Major Authors

Part I. Basic Skills

1.Setup

Zhi John Lu

1.1 Docker

Gang Xu/Yunfan Jin

1.2 Cluster

Gang Xu/Xiaofan Liu/Yunfan Jin

2.Linux

Zhi John Lu

2.1 Basic Command

Xi Hu

2.2 Practice Guide

Xi Hu/Zhuoer Dong

2.3 Linux Bash

Gang Xu

3.R

3.1 R Basics

Zhuoer Dong

3.2 Plot with R

Xiaochen Xi/Zhuoer Dong

4.Python

Yuhuan Tao

PART II. BASIC ANALYSES

1.Blast

Gang Xu

2.Conservation Analysis

Xi Hu

3.Function Analysis

3.1 GO

Gang Xu

3.2 KEGG

Gang Xu

3.3 GSEA

Zhuoer Dong

4.Clinical Analysis

4.1 Survival Analysis

Xiaochen Xi/Yumin Zhu

Part III. NGS DATA ANALYSES

1.Mapping

Meifeng Zhou/Yumin Zhu

1.1 Genome Browser

Xiaofan Liu/Shang Zhang

1.2 bedtools and samtools

Xiaofan Liu/Yunfan Jin

2.RNA-seq

2.1 Expression Matrix

Xiaofan Liu

2.2 Differential Expression with Cufflinks

Meifeng Zhou/Shang Zhang

2.3 Differential Expression with DEseq2 and edgeR

Xinzhe Ni/Shang Zhang

2.4 Alternative Splicing

Zhuoer Dong/Shang Zhang

3.ChIP-seq

Jingyi Cao/Xiaofan Liu

4.Motif

4.1 Sequence Motif

Yumin Zhu

4.2 Structure Motif

Yumin Zhu

5.Network

5.1 Co-expression Network

Xiaochen Xi

5.2 miRNA Targets

Yumin Zhu

5.3 CLIP-seq(RNA-Protein Interactions)

Yumin Zhu/Xiaofan Liu

6.RNA Regulation Analyses

6.1 Alternative Splicing

Zhuoer Dong/Shang Zhang

6.2 APA (Alternative Polyadenylation)

Yumin Zhu

6.3 Chimeric RNA

Yinghui Chen

6.4 RNA Editing

Yumin Zhu

6.5 SNV/INDEL

Yinghui Chen

6.6 RNA Modification

6.7 RNA Degradation

6.8 Translation:Ribo-seq

Yumin Zhu/Weihao Zhao/Xiaofan Liu

6.9 RNA Structure

Yumin Zhu/Xiaofan Liu

Part IV. MACHINE LEARNING

1.Machine Learning Basics

Xiaofan Liu/Xupeng Chen/Zhi John Lu

1.1 Data Pre-processing

Xinzhe Ni/Xiaofan Liu

1.2 Data Visualization & Dimension Reduction

Xinzhe Ni/Xiaofan Liu

1.3 Feature Extraction and Selection

Xinzhe Ni/Xiaofan Liu

1.4 Machine Learning Classifiers/Models

Xinzhe Ni/Xiaofan Liu

1.5 Performance Evaluation

Xiaofan Liu

2.Machine Learning with R

Xupeng Chen/Xiaofan Liu

3.Machine Learning with Python

Xupeng Chen/Xiaofan Liu

Part V. QUIZ

1.Precision Medicine - exSEEK

Xiaofan Liu/Xupeng Chen

2.RNA Regulation - RiboShape

Xiaofan Liu/Yizi Zhao

3.Single Cell Data Analysis

Yu Li

Appendix

Appendix I. Keep Learning

Zhi John Lu

Appendix II. Databases & Servers

Yumin Zhu

Appendix III. How to Backup

Gang Xu/Zhi John Lu

Appendix IV. Teaching Materials

Gang Xu/Xiaofan Liu/Zhi John Lu

Appendix V. Software and Tools

Yumin Zhu