8.cfDNA

Previous cfDNA studies have demonstrated that various features could be derived from cfDNA-seq and cfDNA methylation (cfDNAmet) sequencing data for diagnostic measurement. These features include:

• cfDNAmet-seq

  • methylation level at promoter region

  • methylation level at gene body level

  • ...

• cfDNA-seq

  • Mutation (requires deep sequencing depth)

  • CNV

  • Nucleosome occupancy footprint (scores: ration/CPM/WPS) (Pipelines by Y Li)

  • Fragment size profile

  • End motif pattern

  • ...

• Instructions: Analyze cfDNA features and Related cfDNA publications by PF Bao

• Literatures: cfDNA-seq Analysis - Lu Lab Docs